Class Vp Software Hplc

Description i. Purpose: to provide a procedure to operate and maintain the shimadzu make class vp series gradient hplc system. Scope: this procedure is applicable for the gradient hplc, shimadzu -class vp series used in the quality control department iii. Responsibility: it is the responsibility of all the personnel involved in the analysis using of the system. Arabian Love Poems Nizar Qabbani Pdf Printer.

Document reference: sops: core procedure: calibration of equipment forms: calibration of hplc v. Operation: 1.0 preliminary check: 1.1 check that the instrument is clean and free from dust, if not, clean with a soft cloth duster. 2.0 the lc-10 avp series hplc work station consists of: 2.1. Lc-10at vp pump 2.2. Spd-10 avp uv vis detector 2.3. Impressora Hp D1560 Driver on this page.

Sil-ht- a system controller and auto sampler 2.4. Computer with class vp software version 6.10 3.0 basic operation: 3.1. Switch ‘on’ the main for lc-10 pump 3.2. Switch ‘on’ the main for auto injector 3.3. Switch ‘on’ the main for uv vis detector 3.4. Switch ‘on’ the system controller and the computer.

After switching ‘on’ all the components, finally switch ‘on’ the computer. Default screen appears on the respective screen modules. Double click on the (shimadzu class vp) icon in the screen. Double click on instrument 1 icon. Here for the beep sound, which indicates the communication between the system and the software.

Canon K10339 Driver. We purchased a Shimadzu HPLC with PDA detector about 12 months ago running Class VP 5.02 software (recently upgraded to 5.03) under Windows 95. Unlike some reports I have read, we have found the hardware to be very reliable, easy to use and the results are reproducible. The Class VP software. Apr 17, 2013  An overview of the procedures for generating a multipoint calibration in CLASS-VP or EZStart version 7.x.

Operating procedure for lc-10avp pump 3.10. Open the drain valve of the pump. Press the purge key.

Ensure that there are no air bubbles in the flow line and press purge/pump key to stop purging. Close the drain valve of the pump. System software Driver Printer Hp 710c Windows 7 here. 3.11 check the method name displayed on the top of the screen. 3.12 click on the method option, click the instrument set up. Click on the pump, set the a.

Flow in ml/min. P.max in kgf/cm2. 3.13 click on the detector spd-10 avp, icon. Set the wavelength in nm and run time in min. and save the method.

3.14 after setting the above parameters, click on download at the bottom of the screen. 3.15 observe for the message displayed on the screen.

Click the ok icon and close the screen. This indicates the software successfully downloaded the set information to the system. 3.16 click on sequence. Click on edit, edit table will appear. Set the vial number. Repetitions from the vial (no. Of injections) sample id, file name and finally description.

Autozero/purge injector / rinse injector/instrument ‘on/off’ 3.17 go to control option, click the direct control. Direct control message will appear on the top left corner of the screen. Click on the instrument on/off icon. It activates the whole system act led will glow on the system controller. Before injecting a sample observe for the base line by pressing preview run in control option. To start a run, press the single run into the control option or blue arrow icon on the main screen. If the injections are in sequence mode, press the double green arrow button on the main screen.

To stop the single run, press the stop run column in the control option. Results & printouts 3.21 click on the shimadzu class-vp button. 3.22 double click on the instrument off line processing instrument 1 will appear.

Double click instrument 1. 3.23 on double click, instrument 1 (off line). The method used and the data file namewill appear on the top of the screen. 3.24 to open, select data file, click file, under file option, click data, press open, data files will appear. Select the data and press open, chromatogram will appear on the screen.

3.25 to check the results, press view and then method custom report. 3.26 class-vp custom report will appear on the screen. 3.27 to print click the right button of the mouse and press print. Calibration calibration frequency: once in three months 1.0 calibration of the pump 1.1 check the flow rate of the filtered and degassed purified water as follows: 1.2 disconnect any column if connected to the system and connect restriction capillary 1.3 set the flow of the pump at 1.0 ml per minute.

Keep the system at this flow rate for about 5 minutes to equilibrate the system. 1.4 collect the volume of water delivered for 5 minutes and determine the weight of the water. 1.5 convert the weight in to volume by the following table: temperature, volume, in ml, temperature, volume, in ml, °c of 1g of water °c of 1g of water 20 1.0028 26 1.0042 21 1.003 27 1.0045 22 1.0032 28 1.0047 23 1.00345 29 1.005 24 1.0037 30 1.0053 25 1.0039 flow = volume obtained / time measured 1.6 repeat the above for 2 and 3 ml flow rates. 1.7 acceptance criteria: flow rate: + 0.1ml 2.0 column compartment thermostat / oven calibration. 2.1 disconnect any column if connected to the system. 2.2 purge the system with purified water to remove any solvents and previous buffer salts. 2.3 connect a restricted capillary of dimension 2m x 0.12 mm id in place of column.